4YU3
The crystal structure of mongoose (Helogale parvula) hemoglobin at pH 8.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-04-23 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5417 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 86.038, 88.947, 55.600 |
Unit cell angles | 90.00, 115.39, 90.00 |
Refinement procedure
Resolution | 29.820 - 2.450 |
R-factor | 0.26708 |
Rwork | 0.264 |
R-free | 0.29820 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.843 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 16749 |
<I/σ(I)> | 13.2 |
Completeness [%] | 99.0 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 293 | 25% PEG 3350, 50mM Tris-HCl |