4YTV
Crystal structure of Mdm35
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0, 0.97919, 0.97932, 0.96426 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 59.144, 59.144, 123.277 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.570 - 1.450 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.075 | 0.624 |
| Number of reflections | 15031 | |
| <I/σ(I)> | 68.5 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.9 | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M MES pH 6.5, 1.2 M Ammonium sulfate, 10 mM Cobalt(II) chloride |
