4YTK
Structure of the KOW1-Linker1 domain of Transcription Elongation Factor Spt5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.5484, 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.693, 44.394, 64.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.490 - 1.090 |
R-factor | 0.1831 |
Rwork | 0.182 |
R-free | 0.19940 |
Structure solution method | SAD |
Starting model (for MR) | experimental |
RMSD bond length | 0.009 |
RMSD bond angle | 1.321 |
Data reduction software | HKL-2000 (not known) |
Data scaling software | HKL-2000 (not known) |
Phasing software | PHENIX (not known) |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.500 | 1.110 |
High resolution limit [Å] | 1.090 | 1.090 |
Rmerge | 0.115 | 0.337 |
Number of reflections | 48357 | |
<I/σ(I)> | 14 | 2.6 |
Completeness [%] | 93.9 | 52.1 |
Redundancy | 6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1 M Tris-HCl, pH 8.0, 30% (w/v) PEG 3350 |