4YTE
N-terminal domain of HmdIII from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-31 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.971 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.780, 53.780, 127.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.575 - 2.150 |
| R-factor | 0.2271 |
| Rwork | 0.226 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | N-terminal domain of HmdII from M. jannaschii |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.669 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 | |
| High resolution limit [Å] | 2.150 | 12.000 | 2.150 |
| Rmerge | 0.101 | 0.037 | 0.693 |
| Rmeas | 0.107 | 0.040 | 0.775 |
| Total number of observations | 99182 | ||
| Number of reflections | 12012 | 98 | 1364 |
| <I/σ(I)> | 17.08 | 47.59 | 2.5 |
| Completeness [%] | 98.4 | 98 | 90.6 |
| Redundancy | 8.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281 | 20 % (w/v) PEG3350, 0.2M sodium thiocyanate |
