4YTD

Crystal structure of the C-terminal Coiled Coil of mouse Bicaudal D1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-16
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97924
Spacegroup name	C 1 2 1
Unit cell lengths	59.029, 36.825, 104.303
Unit cell angles	90.00, 99.83, 90.00

Refinement procedure

Resolution	31.113 - 1.500
R-factor	0.1765
Rwork	0.175
R-free	0.20620
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.308
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	35669
<I/σ(I)>	44.6
Completeness [%]	99.6
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	277	48% MPD, 0.1 M Tris-HCL, 0.2 M ammonium phosphate, 10 mM TCEP