4YT8
SeMet-labelled HmdII from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.570, 77.970, 153.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.588 - 1.902 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21510 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.193 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 | |
| High resolution limit [Å] | 1.900 | 12.000 | 1.900 |
| Rmerge | 0.078 | 0.065 | 0.586 |
| Rmeas | 0.127 | 0.072 | 0.386 |
| Total number of observations | 276025 | ||
| Number of reflections | 50381 | 385 | 16577 |
| <I/σ(I)> | 11.8 | 35.26 | 2.22 |
| Completeness [%] | 99.3 | 98 | 95.7 |
| Redundancy | 5.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 281 | 35% (v/v) PEG 300 and 100 mM phosphate-citrate buffer |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 281 | 35% (v/v) PEG 300 and 100 mM phosphate-citrate buffer |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 281 | 35% (v/v) PEG 300 and 100 mM phosphate-citrate buffer |
