4YST
Structure of copper nitrite reductase from Geobacillus thermodenitrificans - 24.9 MGy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.75000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 115.177, 115.177, 84.376 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.120 - 1.340 |
| R-factor | 0.15635 |
| Rwork | 0.155 |
| R-free | 0.18510 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.340 |
| Number of reflections | 88348 |
| <I/σ(I)> | 8.77 |
| Completeness [%] | 93.1 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 0.1M sodium acetate, 5.5%(w/v) PEG 4000, 0.075M CuSO4 |
