4YSI
Structure of USP7 with a novel viral protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-16 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 69.975, 69.975, 45.444 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.294 - 1.020 |
R-factor | 0.1541 |
Rwork | 0.154 |
R-free | 0.16000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yy6 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.909 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.8) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.480 | 49.480 | 1.100 |
High resolution limit [Å] | 1.020 | 2.700 | 1.020 |
Rmerge | 0.039 | 0.031 | 0.531 |
Rpim | 0.017 | 0.013 | 0.246 |
Total number of observations | 684942 | 40554 | 125204 |
Number of reflections | 111563 | ||
<I/σ(I)> | 20.6 | 57.2 | 3 |
Completeness [%] | 99.8 | 99.9 | 99.3 |
Redundancy | 6.1 | 6.5 | 5.5 |
CC(1/2) | 0.999 | 0.999 | 0.888 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30% PEG4K, 0.2M LiSO4, 0.1M TrisHCl |