4YSI

Structure of USP7 with a novel viral protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	69.975, 69.975, 45.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.294 - 1.020
R-factor	0.1541
Rwork	0.154
R-free	0.16000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yy6
RMSD bond length	0.006
RMSD bond angle	0.909
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.8)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.480	49.480	1.100
High resolution limit [Å]	1.020	2.700	1.020
Rmerge	0.039	0.031	0.531
Rpim	0.017	0.013	0.246
Total number of observations	684942	40554	125204
Number of reflections	111563
<I/σ(I)>	20.6	57.2	3
Completeness [%]	99.8	99.9	99.3
Redundancy	6.1	6.5	5.5
CC(1/2)	0.999	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	30% PEG4K, 0.2M LiSO4, 0.1M TrisHCl