4YSD
Room temperature structure of copper nitrite reductase from Geobacillus thermodenitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 300 |
Detector technology | CCD |
Collection date | 2013-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90000 |
Spacegroup name | H 3 |
Unit cell lengths | 116.197, 116.197, 85.553 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.530 - 1.350 |
R-factor | 0.09795 |
Rwork | 0.097 |
R-free | 0.12067 |
RMSD bond length | 0.028 |
RMSD bond angle | 2.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.350 |
Number of reflections | 94321 |
<I/σ(I)> | 24.4 |
Completeness [%] | 99.8 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5.5% PEG 4000, 0.1M sodium acetate, 0.075M copper sulphate, pH 4.5 |