4YSB

Crystal structure of ETHE1 from Myxococcus xanthus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	CCD
Collection date	2014-08-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00001
Spacegroup name	P 61
Unit cell lengths	116.603, 116.603, 65.061
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.419 - 2.502
R-factor	0.1799
Rwork	0.175
R-free	0.22620
RMSD bond length	0.003
RMSD bond angle	0.595
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	17540
<I/σ(I)>	33.625
Completeness [%]	99.9
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	200 mM Calcium chloride, 100 mM HEPES (pH 7.5), 15% glycerol, and 15% (w/v) PEG