4YSA
Completely oxidized structure of copper nitrite reductase from Geobacillus thermodenitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2014-02-12 |
Detector | MPCCD |
Wavelength(s) | 1.129 |
Spacegroup name | H 3 |
Unit cell lengths | 116.197, 116.197, 85.553 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.750 - 1.430 |
R-factor | 0.13734 |
Rwork | 0.137 |
R-free | 0.14915 |
RMSD bond length | 0.029 |
RMSD bond angle | 2.385 |
Data reduction software | CrystFEL |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.800 |
High resolution limit [Å] | 1.430 |
Number of reflections | 79590 |
<I/σ(I)> | 3.4 |
Completeness [%] | 100.0 |
Redundancy | 245.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5.5% PEG 4000, 0.1M sodium acetate pH 4.5, 0.075M copper sulphate |