4YS5
DNA sequence containing 2'-Se-dC modification
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-12-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.987 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 41.621, 41.621, 25.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.080 - 1.650 |
R-factor | 0.22695 |
Rwork | 0.225 |
R-free | 0.26294 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.929 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 2939 | |
<I/σ(I)> | 52.9 | 3.16 |
Completeness [%] | 99.7 | 97.3 |
Redundancy | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% MPD, sodium cacodylate, sodium chloride, barium chloride |