4YS0

Conformational changes of the clamp of the protein translocation ATPase SecA from Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	CCD
Collection date	2011-07-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97914
Spacegroup name	P 21 21 21
Unit cell lengths	64.404, 110.160, 115.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.920 - 1.897
R-factor	0.181
Rwork	0.179
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jux
RMSD bond length	0.009
RMSD bond angle	1.111
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	39.920
High resolution limit [Å]	1.897
Number of reflections	65151
<I/σ(I)>	13.21
Completeness [%]	98.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	imidazole, pH 7.0 methyl-2,4-pentanediol