4YRV
Crystal structure of Anabaena transcription factor HetR complexed with 21-bp DNA from hetP promoter
Replaces: 4K1MExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97886 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.559, 90.559, 242.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.950 - 2.800 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hri |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.082 | 0.535 |
| Number of reflections | 25553 | |
| <I/σ(I)> | 28.7 | 6.8 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 14.1 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | 0.3M calcium acetate, 0.1M Bicine |
