4YRU

Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979490
Spacegroup name	P 21 21 21
Unit cell lengths	72.680, 88.800, 100.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.590 - 2.800
R-factor	0.2481
Rwork	0.247
R-free	0.29090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2you
RMSD bond length	0.003
RMSD bond angle	0.747
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.590	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.124	0.374
Number of reflections	16258
<I/σ(I)>	1.1
Completeness [%]	98.0	98.2
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1M MES pH6.0, 200mM NaCl,16% (w/v) PEG 6K