4YRA
mouse TDH in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.445, 157.310, 162.336 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 44.350 - 2.650 |
| R-factor | 0.2196 |
| Rwork | 0.217 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yr9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 5.710 | 2.650 |
| Rmerge | 0.062 | 0.027 | 0.387 |
| Rmeas | 0.081 | 0.035 | 0.508 |
| Rpim | 0.051 | 0.021 | 0.325 |
| Total number of observations | 266100 | ||
| Number of reflections | 127736 | ||
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 93.9 | 93.1 | 94.6 |
| Redundancy | 2.1 | 2.1 | 2 |
| CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.1 M Sodium HEPES, 10%(w/v) PEG 4000, 10%(v/v) 2-Propanol |
