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4YQW

Mutant Human DNA Polymerase Eta Q38A/R61A Inserting dCTP Opposite Template G

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-G
Synchrotron siteAPS
Beamline21-ID-G
Temperature [K]100
Detector technologyCCD
Collection date2014-06-21
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.97856
Spacegroup nameP 61
Unit cell lengths99.238, 99.238, 82.378
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution42.971 - 2.064
R-factor0.177
Rwork0.174
R-free0.22700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4o3n
RMSD bond length0.008
RMSD bond angle1.108
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: dev_1839))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.100
High resolution limit [Å]2.0602.060
Rmerge0.1490.964
Number of reflections28389
<I/σ(I)>10.21.29
Completeness [%]99.285.6
Redundancy5.53.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6291NaMES (pH 6.0), polyethylene glycol monomethyl ether 2000, and calcium chloride

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