4YQW
Mutant Human DNA Polymerase Eta Q38A/R61A Inserting dCTP Opposite Template G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 61 |
Unit cell lengths | 99.238, 99.238, 82.378 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.971 - 2.064 |
R-factor | 0.177 |
Rwork | 0.174 |
R-free | 0.22700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.008 |
RMSD bond angle | 1.108 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.149 | 0.964 |
Number of reflections | 28389 | |
<I/σ(I)> | 10.2 | 1.29 |
Completeness [%] | 99.2 | 85.6 |
Redundancy | 5.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | NaMES (pH 6.0), polyethylene glycol monomethyl ether 2000, and calcium chloride |