4YQF

GTPase domain of Human Septin 9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	57.040, 77.523, 77.909
Unit cell angles	90.00, 105.81, 90.00

Refinement procedure

Resolution	19.726 - 2.730
R-factor	0.2096
Rwork	0.208
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Unpublished high resolution model
RMSD bond length	0.003
RMSD bond angle	0.827
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.890
High resolution limit [Å]	2.730	7.630	2.730
Rmerge	0.122	0.026	0.681
Rmeas	0.143	0.031	0.807
Total number of observations	61083
Number of reflections	17271	789	2606
<I/σ(I)>	10.57	36.3	1.92
Completeness [%]	98.4	93.2	93.5
Redundancy	3.5		3.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291	24% PEG 1500, 20% glycerol