4YPI
Structure of Ebola virus nucleoprotein N-terminal fragment bound to a peptide derived from Ebola VP35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.990 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 149.752, 194.818, 347.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.710 |
| R-factor | 0.2546 |
| Rwork | 0.253 |
| R-free | 0.28500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.720 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.115 | 0.242 |
| Number of reflections | 23161 | |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 84.7 | 10.2 |
| Redundancy | 4.3 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | streak seeding in well solution containing 100 mM Tris pH 7.2, 50 mM Hepes pH 7, and 23% PEG400 |
