4YPC

Trimeric crystal structure of vimentin coil1B fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	H 3
Unit cell lengths	35.447, 35.447, 200.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.390 - 1.440
R-factor	0.2533
Rwork	0.248
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3swk 3uf1
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		1.480
High resolution limit [Å]	1.440	6.440	1.440
Rmerge	0.069	0.059	0.567
Rmeas	0.073	0.064	0.658
Total number of observations	177342
Number of reflections	16952	175	1276
<I/σ(I)>	19.94	33.51	2.32
Completeness [%]	99.7	87.9	99.7
Redundancy	10.46		3.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	protein in 10 mM Tris pH 8, 38 mM NaCl mixed in ratio 1:1 with 1M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5
1	VAPOR DIFFUSION, SITTING DROP	6.6	298	protein in 10 mM Tris pH 8, 38 mM NaCl mixed in ratio 1:1 with 1M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5