4YOX
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-01-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.737, 94.755, 105.154 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.761 - 2.050 |
R-factor | 0.1803 |
Rwork | 0.179 |
R-free | 0.20750 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.167 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.392 | 19.761 | 2.160 |
High resolution limit [Å] | 2.050 | 6.480 | 2.050 |
Rmerge | 0.025 | 0.867 | |
Rmeas | 0.084 | ||
Rpim | 0.031 | 0.010 | 0.342 |
Total number of observations | 435374 | 12565 | 64361 |
Number of reflections | 60017 | ||
<I/σ(I)> | 16.2 | 54.7 | 2.5 |
Completeness [%] | 99.9 | 96.6 | 100 |
Redundancy | 7.3 | 6.3 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 293 | MES, PEG6000, LiCl |