4YOW
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-01-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.569, 100.265, 113.181 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.983 - 2.500 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.24040 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.718 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 75.051 | 19.983 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.027 | 0.958 | |
| Rmeas | 0.088 | ||
| Rpim | 0.033 | 0.011 | 0.415 |
| Total number of observations | 222012 | 6972 | 27399 |
| Number of reflections | 32549 | ||
| <I/σ(I)> | 15.9 | 50 | 2.2 |
| Completeness [%] | 99.3 | 93.9 | 97.2 |
| Redundancy | 6.8 | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | MES, PEG6000, LiCl |
