4YOW
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 in complex with poly-dC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-01-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.569, 100.265, 113.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.983 - 2.500 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24040 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.718 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 75.051 | 19.983 | 2.640 |
High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
Rmerge | 0.027 | 0.958 | |
Rmeas | 0.088 | ||
Rpim | 0.033 | 0.011 | 0.415 |
Total number of observations | 222012 | 6972 | 27399 |
Number of reflections | 32549 | ||
<I/σ(I)> | 15.9 | 50 | 2.2 |
Completeness [%] | 99.3 | 93.9 | 97.2 |
Redundancy | 6.8 | 6.4 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | MES, PEG6000, LiCl |