4YOU
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 2.20A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2008-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.940, 88.980, 93.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.963 - 2.200 |
R-factor | 0.1953 |
Rwork | 0.193 |
R-free | 0.23290 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.891 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.260 | |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.067 | 0.024 | 0.546 |
Rmeas | 0.074 | 0.026 | 0.639 |
Total number of observations | 196045 | ||
Number of reflections | 35388 | 393 | 2530 |
<I/σ(I)> | 18.28 | 60.82 | 2.01 |
Completeness [%] | 99.5 | 85.2 | 97.6 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | CaCl2, Bis-Tris, Polyethylene glycol monomethyl ether 550 |