4YOT
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 2.15A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-10-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.610, 94.820, 99.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.573 - 2.150 |
| R-factor | 0.1843 |
| Rwork | 0.182 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.210 | |
| High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
| Rmerge | 0.084 | 0.043 | 0.634 |
| Rmeas | 0.091 | 0.047 | 0.720 |
| Total number of observations | 337105 | ||
| Number of reflections | 49267 | 548 | 3559 |
| <I/σ(I)> | 13.05 | 26.98 | 2.6 |
| Completeness [%] | 99.7 | 86.7 | 98.6 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | polyethylene glycol monomethyl ether 550 |
