4YOR
Crystal structure of a trimeric exonuclease PhoExo I from Pyrococcus horikoshii OT3 at 1.52A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-05-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 112.065, 112.065, 202.282 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.696 - 1.520 |
R-factor | 0.1607 |
Rwork | 0.160 |
R-free | 0.18040 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 87.501 | 19.696 | 1.600 |
High resolution limit [Å] | 1.520 | 4.810 | 1.520 |
Rmerge | 0.018 | 0.536 | |
Rmeas | 0.035 | ||
Rpim | 0.013 | 0.007 | 0.332 |
Total number of observations | 490933 | 18205 | 30293 |
Number of reflections | 74001 | ||
<I/σ(I)> | 30.2 | 87.9 | 2.1 |
Completeness [%] | 98.6 | 96.9 | 91.1 |
Redundancy | 6.6 | 7.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | PEG 6000 |