4YOF
DosS GAFA Domain Reduced Nitric Oxide Bound Crystal Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.978550 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.593, 78.847, 110.645 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.137 - 1.900 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.22430 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.193 |
Data scaling software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.053 | 0.620 |
Number of reflections | 23788 | |
<I/σ(I)> | 16.7 | 2.5 |
Completeness [%] | 99.1 | 98.8 |
Redundancy | 4.1 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 16-20% (wt/vol) PEG 4000, 0.2M calcium acetate, 0.1M Tris-HCl |