4YOE
Structure of UP1 bound to RNA 5'-AGU-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-22 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.557, 64.523, 141.352 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.340 - 1.920 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l3k |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.950 |
| High resolution limit [Å] | 1.920 | 5.210 | 1.920 |
| Rmerge | 0.071 | 0.046 | 0.463 |
| Total number of observations | 203025 | ||
| Number of reflections | 14648 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 100.0 | 100 | 99.4 |
| Redundancy | 13.9 | 12.7 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 2.5 M ammonium sulfate, 50 mM MES pH 5.6, and 10 mM magnesium acetate |
