4YOE

Structure of UP1 bound to RNA 5'-AGU-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-22
Detector	RAYONIX MX-300
Wavelength(s)	0.9786
Spacegroup name	C 2 2 21
Unit cell lengths	40.557, 64.523, 141.352
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.340 - 1.920
R-factor	0.188
Rwork	0.186
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3k
RMSD bond length	0.010
RMSD bond angle	1.060
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.920	5.210	1.920
Rmerge	0.071	0.046	0.463
Total number of observations	203025
Number of reflections	14648
<I/σ(I)>	9.4
Completeness [%]	100.0	100	99.4
Redundancy	13.9	12.7	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	277	2.5 M ammonium sulfate, 50 mM MES pH 5.6, and 10 mM magnesium acetate