4YO5
EAEC T6SS TssA-Cterminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 112.280, 90.630, 120.090 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 46.200 - 3.350 |
| R-factor | 0.2265 |
| Rwork | 0.225 |
| R-free | 0.25970 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 3.450 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.120 | 0.760 |
| Number of reflections | 33982 | |
| <I/σ(I)> | 8.2 | 2 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 300 nL of protein at 4.7 mg/mL with 100 nL of 20% PEG 8000, 0.2M Calcium Acetate, 0.1 M MES pH 6.8 |
