4YO0

Crystal structure of monoclonal anti-human podoplanin antibody NZ-1 with bound PA peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.980
Spacegroup name	P 1 21 1
Unit cell lengths	42.180, 80.010, 132.520
Unit cell angles	90.00, 96.61, 90.00

Refinement procedure

Resolution	40.000 - 1.560
R-factor	0.1808
Rwork	0.179
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yny
RMSD bond length	0.018
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.650
High resolution limit [Å]	1.560	1.560
Number of reflections	121358
<I/σ(I)>	17.5	1.5
Completeness [%]	97.2	90.2
Redundancy	7.5	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, sodium citrate