4YNV
Assembly Chaperone of RpL4 (Acl4) (Residues 28-338)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97936, 0.97961, 0.94937 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.487, 49.103, 80.051 |
| Unit cell angles | 98.66, 97.88, 100.02 |
Refinement procedure
| Resolution | 19.909 - 2.950 |
| R-factor | 0.2288 |
| Rwork | 0.227 |
| R-free | 0.25440 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.698 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 30229 | |
| <I/σ(I)> | 22.4 | 1.9 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20 % (w/v) PEG 3350 0.2 M potassium formate |
