4YNV
Assembly Chaperone of RpL4 (Acl4) (Residues 28-338)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97936, 0.97961, 0.94937 |
Spacegroup name | P 1 |
Unit cell lengths | 49.487, 49.103, 80.051 |
Unit cell angles | 98.66, 97.88, 100.02 |
Refinement procedure
Resolution | 19.909 - 2.950 |
R-factor | 0.2288 |
Rwork | 0.227 |
R-free | 0.25440 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.698 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 30229 | |
<I/σ(I)> | 22.4 | 1.9 |
Completeness [%] | 99.0 | 98.3 |
Redundancy | 4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 20 % (w/v) PEG 3350 0.2 M potassium formate |