4YN2
THE ATOMIC STRUCTURE OF WISEANA SPP ENTOMOPOXVIRUS (WSEPV) FUSOLIN SPINDLES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.182, 68.182, 128.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.360 - 2.020 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ACEPV FUSOLIN (UNPUBLISHED) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.060 | 2.130 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.183 | 0.537 |
| Number of reflections | 20209 | |
| <I/σ(I)> | 8.7 | 3.8 |
| Completeness [%] | 99.0 | 95.8 |
| Redundancy | 8.3 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 7 | 300 | NATURALLY OCCURRING INTRACELLULAR FUSOLIN MICRO-CRYSTALS WERE PURIFIED FROM EPV-INFECTED LARVAE OF WISEANA SPP. MOTHS |
