4YN1
THE ATOMIC STRUCTURE OF ANOMALA CUPREA ENTOMOPOXVIRUS (ACEPV) FUSOLIN SPINDLES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.887, 71.887, 127.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.680 - 1.900 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bem |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.680 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.191 | 0.699 |
| Number of reflections | 27160 | |
| <I/σ(I)> | 12.7 | 4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.5 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 7 | 300 | NATURALLY OCCURRING INTRACELLULAR FUSOLIN MICRO-CRYSTALS WERE PURIFIED FROM EPV-INFECTED LARVAE OF ANOMALA CUPREA MOTHS |
