4YM8

Crystal structure of hen egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2015-01-30
Detector	MPCCD
Wavelength(s)	1.77
Spacegroup name	P 43 21 2
Unit cell lengths	79.200, 79.200, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.001 - 2.100
R-factor	0.163
Rwork	0.161
R-free	0.17890
RMSD bond length	0.008
RMSD bond angle	1.025
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.100
Number of reflections	15532
<I/σ(I)>	29
Completeness [%]	100.0
Redundancy	364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	sodium chloride, PEG 6000, pH 3.0, batch, temperature 293K