4YM6
Crystal structure of the human nucleosome containing 6-4PP (outside)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-30 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.713, 109.483, 178.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.831 - 3.514 |
R-factor | 0.2464 |
Rwork | 0.244 |
R-free | 0.28680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.019 |
RMSD bond angle | 1.336 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
Rmerge | 0.096 | 0.043 | 0.551 |
Total number of observations | 174199 | ||
Number of reflections | 26130 | ||
<I/σ(I)> | 9.3 | 4.205 | |
Completeness [%] | 99.6 | 96.5 | 100 |
Redundancy | 6.7 | 5.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |