4YM5
Crystal structure of the human nucleosome containing 6-4PP (inside)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-31 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.193, 109.356, 174.417 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.234 - 4.005 |
| R-factor | 0.2067 |
| Rwork | 0.203 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3afa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.354 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.140 |
| High resolution limit [Å] | 4.000 | 8.600 | 4.000 |
| Rmerge | 0.088 | 0.044 | 0.453 |
| Total number of observations | 107264 | ||
| Number of reflections | 17241 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.6 | 96.1 | 100 |
| Redundancy | 6.2 | 5.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
