4YLR

Tubulin Glutamylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	C 2 2 21
Unit cell lengths	78.548, 121.762, 129.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.288 - 2.550
R-factor	0.2267
Rwork	0.222
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.563
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.124	0.066	0.482
Rmeas	0.137	0.073	0.549
Rpim	0.056	0.029	0.256
Total number of observations	106539
Number of reflections	19556
<I/σ(I)>	7.8
Completeness [%]	93.0	89.8	79.4
Redundancy	5.5	5.8	3.9
CC(1/2)		0.994	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG 8000, magnesium acetate