4YLO

E. coli Transcription Initiation Complex - 16-bp spacer and 4-nt RNA

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
格子定数 [Å]	240.888, 208.174, 256.323
格子定数 [度]	90.00, 119.31, 90.00

精密化法

残基	39.950 - 6.000
R因子	0.23151
Rwork	0.227
R-free	0.31429
結合長の平均二乗偏差(RMSD) [Å]	0.012
結合角の平均二乗偏差(RMSD) [度]	1.611
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Quality characteristics

	Overall
分解能 [Å] (低)	40.000
分解能 [Å] (高)	6.000
独立反射数	55356
<I/σ(I)>	13.4
完全性 [%]	99.9
冗長性	5.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7	298	7% PEG3350, 150 mM magnesium chloride, 100 mM HEPES sodium, pH 7.0