4YLB

Crystal Structure of A102D mutant of hsp14.1 from Sulfolobus solfatataricus P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97913
Spacegroup name	H 3 2
Unit cell lengths	132.793, 132.793, 213.058
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.589 - 2.501
R-factor	0.1985
Rwork	0.196
R-free	0.24110
RMSD bond length	0.010
RMSD bond angle	1.179
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.490
Number of reflections	24402
<I/σ(I)>	21.5
Completeness [%]	96.6
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	magnesium sulfate heptahydrate, polyethylene glycol 3350