4YL9

Crystal Structure of wild-type of hsp14.1 from Sulfolobus solfatataricus P2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	65.733, 39.108, 119.674
Unit cell angles	90.00, 91.64, 90.00

Refinement procedure

Resolution	39.875 - 2.353
R-factor	0.2335
Rwork	0.233
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3aab
RMSD bond length	0.009
RMSD bond angle	0.931
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	25552
<I/σ(I)>	13.6
Completeness [%]	99.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	calcium chloride dehydrate, 2-methyl-2,4-pentanediol, bis-tris (pH 6.5), ethylenediaminetetraacetic acid disodium salt dihydrate