4YL7
Crystal structure of the indole prenyltransferase MpnD from Marinactinospora thermotolerans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2013-11-02 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.660, 53.700, 132.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.139 - 1.601 |
R-factor | 0.1813 |
Rwork | 0.180 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yzj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.127 |
Data reduction software | XDS (XDS-OSX10.9.4_Darwin13.3.0) |
Data scaling software | XSCALE (XDS-OSX10.9.4_Darwin13.3.0) |
Phasing software | MOLREP (CCP4-6.4.0) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.049 | 0.315 |
Number of reflections | 44641 | |
<I/σ(I)> | 25.04 | 5.83 |
Completeness [%] | 99.6 | 98.3 |
Redundancy | 7.18 | 6.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris-HCl (pH8.5), 1470 mM (NH4)2SO4 |