4YL2
Aerococcus viridans L-lactate oxidase Y191F mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.852, 119.081, 106.810 |
| Unit cell angles | 90.00, 121.30, 90.00 |
Refinement procedure
| Resolution | 37.530 - 1.900 |
| R-factor | 0.18459 |
| Rwork | 0.183 |
| R-free | 0.22847 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.866 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.270 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.269 |
| Number of reflections | 422607 | |
| <I/σ(I)> | 24.8 | 5 |
| Completeness [%] | 99.7 | 95.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% PEG 3000 50 mM Tris, 50 mM pyruvate |
