4YKZ
Heat Shock Protein 90 Bound to CS320
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 64.908, 88.340, 99.387 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.010 - 1.850 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.20590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | In-house APO Structure |
RMSD bond length | 0.010 |
RMSD bond angle | 0.990 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | BUSTER-TNT (BUSTER 2.11.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.570 |
High resolution limit [Å] | 1.540 | 4.180 | 1.540 |
Rmerge | 0.058 | 0.034 | 0.383 |
Total number of observations | 297686 | ||
Number of reflections | 42245 | ||
<I/σ(I)> | 14 | ||
Completeness [%] | 99.5 | 91.8 | 100 |
Redundancy | 7 | 6.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 26% Peg 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.0, Cryo conditions: 35% Peg 4000, 10% glycerol |