4YKG
Crystal Structure of the Alkylhydroperoxide Reductase subunit F (AhpF) with NAD+ from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.882, 59.576, 121.508 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 27.000 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.518 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.108 | 0.648 |
| Number of reflections | 26052 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 91.9 | 90.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M Na-Hepes, 2.5 % (v/v) PEG 400, 2 M ammonium sulfate, 10 mM cadmium chloride |
