4YKC

Crystal structure of cerebral cavernous malformation 2 C-terminal adaptor domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97916
Spacegroup name	I 41 2 2
Unit cell lengths	113.300, 113.300, 102.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.187 - 2.700
R-factor	0.2212
Rwork	0.219
R-free	0.26670
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.701
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.410	2.850
High resolution limit [Å]	2.700	2.700
Rmerge		0.381
Number of reflections	9435
<I/σ(I)>	23.1	6.9
Completeness [%]	99.7	100
Redundancy	14.3	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	45% Tacsimate