4YKA

The structure of Agrobacterium tumefaciens ClpS2 in complex with L-tyrosinamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-18
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.945, 92.648, 94.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.133 - 2.801
R-factor	0.1899
Rwork	0.187
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yjm
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.133
High resolution limit [Å]	2.801
Number of reflections	13019
<I/σ(I)>	15.7
Completeness [%]	99.7
Redundancy	12.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	2.2M Ammonium Sulfate 0.2M Sodium Formate