4YK9

Complex structure of BCL-XL and mutated BIM BH3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-06
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 3
Unit cell lengths	81.738, 81.738, 42.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.633 - 1.700
R-factor	0.1941
Rwork	0.192
R-free	0.22750
RMSD bond length	0.007
RMSD bond angle	1.177
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.650
Number of reflections	37932
<I/σ(I)>	2.08
Completeness [%]	99.2
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	287	0.1 M sodium acetate (pH 4.6), 0.01 M calcium chloride and 15% v/v 2-methyl-2,4-pentanediol