4YJZ

Human antibody H2526 in complex with influenza hemagglutinin H1 Solomon Islands/03/2006

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.99998
Spacegroup name	I 2 2 2
Unit cell lengths	63.149, 153.166, 177.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.703 - 2.720
R-factor	0.2312
Rwork	0.230
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbn 4hkx
RMSD bond length	0.011
RMSD bond angle	1.405
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.720	2.720
Rmerge	0.070	0.648
Number of reflections	23649
<I/σ(I)>		2.892
Completeness [%]	99.8	100
Redundancy	5.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M HEPES, 30% PEG 400 and 0.1M ammonium sulfate