4YJX
The structure of Agrobacterium tumefaciens ClpS2 bound to L-phenylalaninamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-17 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.033, 92.997, 94.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.864 - 2.547 |
| R-factor | 0.1771 |
| Rwork | 0.175 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yjm |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.813 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.864 | 2.590 |
| High resolution limit [Å] | 2.547 | 2.550 |
| Rmerge | 0.501 | |
| Number of reflections | 17257 | |
| <I/σ(I)> | 19.5 | 3.08 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 2.2M Ammonium Sulfate 0.2M Sodium Formate |
