4YJS
THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000226
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-03 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.407, 84.617, 39.756 |
| Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.220 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.22570 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | In-house SYK model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM (6.2.4) |
| Data scaling software | SCALA (3.2.5) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.712 | 39.103 | 2.340 |
| High resolution limit [Å] | 2.220 | 7.020 | 2.220 |
| Rmerge | 0.059 | 0.027 | 0.486 |
| Total number of observations | 45294 | 1479 | 6459 |
| Number of reflections | 12409 | ||
| <I/σ(I)> | 18.7 | 59.5 | 2.3 |
| Completeness [%] | 100.0 | 98.7 | 100 |
| Redundancy | 3.7 | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 32.5% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.8, 5mM DTT |
