4YJO

THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000222

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97935
Spacegroup name	P 1 21 1
Unit cell lengths	39.936, 84.821, 40.412
Unit cell angles	90.00, 99.24, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.1862
Rwork	0.184
R-free	0.22990
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	in-house SYK model
RMSD bond length	0.011
RMSD bond angle	1.458
Data reduction software	MOSFLM (6.2.4)
Data scaling software	SCALA (3.2.5)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.784	39.873	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge	0.048	0.028	0.488
Total number of observations	107987	3705	14710
Number of reflections	30502
<I/σ(I)>	16.3	49.5	2
Completeness [%]	87.6	89.5	82
Redundancy	3.5	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	30% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.8, 5mM TCEP